《植物生理学报》 2012, 48(2): 181-188

生长调节物质和可溶性糖含量对丹参中丹酚酸类物质积累的影响

王春丽^1,2, 梁宗锁^1,*, 李殿荣², 刘岩³, 刘峰华³

1西北农林科技大学生命科学学院, 陕西杨凌712100; 2陕西省杂交油菜研究中心, 陕西大荔715105; 3天津天士力现代中药资

通信作者：梁宗锁；E-mail: liangzs@ms.iswc.ac.cn；Tel: 029-87092373

摘　要：

采用盆栽实验研究了生长调节物质水杨酸(SA)、茉莉酸甲酯(MeJA)、赤霉素(GA3)对不同生长时期丹参植株中非结构糖含量、碳/氮比及根中丹酚酸类物质积累的影响; 并进一步测定培养基中不同浓度蔗糖、葡萄糖、果糖对丹参毛状根中丹酚酸类物质积累的影响, 对盆栽实验的结论进行了验证。结果显示, SA处理的丹参幼苗及花后期植株中蔗糖含量有增加趋势, 而MeJA处理的丹参幼苗及花后期植株及GA3处理的丹参花后期植株中蔗糖积累均有降低趋势; 且SA、MeJA和GA3处理对花后期植株地上和地下部分碳/氮比的影响不同。然而, SA和MeJA处理的丹参幼苗及花后期植株地上部分和根中还原糖含量、GA3处理的花后期植株根中还原糖含量均显著增加; 同时, SA和MeJA处理的丹参幼苗根中迷迭香酸含量,以及SA、MeJA、GA3处理的花后期植株根中迷迭香酸含量和丹酚酸类总量显著增加。毛状根培养结果进一步证明, 葡萄糖促进毛状根中迷迭香酸的产生, 增加丹酚酸类总量, 毛状根中迷迭香酸、丹酚酸B的积累及丹酚酸类总量与培养基中蔗糖浓度不相关。可见, 丹参(植株)根中丹酚酸类物质的产生和积累受SA、MeJA和GA3的诱导, 其与碳/氮比及植株中蔗糖含量没有相关性, 推测植株中葡萄糖含量的增加促进根中丹酚酸类物质的积累。

关键词：丹参; 丹酚酸类物质; 生长调节物质; 蔗糖; 葡萄糖

收稿：2011-10-13   修定：2011-12-29

资助：国家“十一五”支撑计划(2007BAD79B06)和国家科技基础性工作专项重点项目(2007FY110800)。

Effects of Growth-Regulating Substances and Soluble Sugar Contents on Accumulation of Salvianolic Acids in Salvia miltiorrhiza Bunge

WANG Chun-Li^1,2, LIANG Zong-Suo^1,*, LI Dian-Rong², LIU Yan³, LIU Feng-Hua³

¹College of Life Sciences, Northwest A & F University, Yangling, Shaanxi 712100, China; ²Hybrid Rapeseed Research Center of Shaanxi Province, Dali, Shaanxi 715105, China; ³Tianjin Tasly Modern Traditional Chinese Medicine Resources Limited Company, Tianjin 300402, China

Corresponding author: LIANG Zong-Suo; E-mail: liangzs@ms.iswc.ac.cn; Tel: 029-87092373

Abstract:

The study aimed at investigating effects of growth-regulating substances salicylic acid (SA), methyl jasmonate (MeJA) and gibberellic acid (GA3) on accumulation of salvianolic acids, contents of non-structural carbohydrates and carbon/nitrogen ratios in seedlings and fruiting plants of Salvia miltiorrhiza by a pot experiment, further conclusion of which was demonstrated by comparing effects of sucrose, glucose and fructose of different medium concentrations on contents of salvianolic acids in cultures of S. miltiorrhiza hairy roots. The results exhibited an increasing trend in sucrose contents in both the seedlings and fruiting plants of S. miltiorrhiza for the SA treatment; but sucrose contents in both the MeJA-treated seedlings and fruiting plants and in the GA3-treated fruiting plants, together exhibited decreasing trends. Meanwhile, SA, MeJA and GA3 induced various changes in carbon/nitrogen ratios of the fruiting plants. However, contents of reducing sugar in both shoots and roots of the SA- or MeJA-treated different-aged plants and in roots of the GA3-treated fruiting plants, together with contents of rosmarinic acid in roots of the seedlings treated by the SA or MeJA, contents of rosmarinic acid and total amounts of salvianolic acids in roots of the fruiting plants treated by the SA, MeJA or GA3, were significantly increased. The culture experiment further demonstrated that glucose promoted production of rosmarinic acid and enhanced total amount of salvianolic acids in the S. miltiorrhiza hairy roots, whereas contents of salvianolic acids in the cultures did not exhibit positive correlation with medium concentrations of sucrose. This indicates that production and accumulation of salvianolic acids in roots of S. miltiorrhiza plants is induced by SA, MeJA and GA3, which does not correlate with carbon/nitrogen ratio and sucrose content in the plants. It is speculated that enhancement in glucose level of the plant accelerates production of salvianolic acids in the root.

Key words: Salvia miltiorrhiza; salvianolic acids; growth-regulating substances; sucrose; glucose